Figure 5. Effects of vimentin downregulation on myosin light chain phosphorylation.

A, representative immunoblot showing unstimulated or ACh (10−5M, 5 min) stimulated myosin light chain (MLC) phosphorylation in tracheal smooth muscle strips that had been incubated with no ODNs, or with vimentin sense ODNs (S) or vimentin antisense ODNs (AS). NP-MLC, non-phosphorylated MLC; P-MLC, phosphorylated MLC. B, Differences between antisense-treated, sense-treated, and no ODNs-treated strips were not statistically significant (P > 0.05). Values shown are mean ± SE (n = 4–5).