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Release of ATP from Candida caused by incubation with histatin 5 and H2K4b. After C. albicans were incubated for 30 min with and without DIDS, the cells were washed, and then histatin 5 (50 or 100 μg/ml) or H2K4b (50 μg/ml) was added to the culture for 30 min. The amount of released ATP was then measured in each treatment group. Student t test, *, P < 0.05, peptide treatment without DIDS versus. peptide treatment with DIDS.
