FIG. 7.

Colocalization of Dextran and H2K4b in yeast AlexaFluor 594 dexran and floresceinylated H2K4b were incubated with yeast for 2 h, the yeast were then washed with PBS X 2 and then these probes were observed with the LSM510 using 488 nm excitation and 515-545 nm emission filters for fluorescein and 543 nm excitation and 560 nm long pass emission filter for AlexaFluor 594. The fluorescein-labeled polymer (A) and AlexaFluor 594 dextran (B) were shown with the overlaid image (C) to visualized colocalization. The polymer and dextran colocalized to small peripheral endosomal-like vesicles (thin arrows) or to the cytosol compartment (thick arrows).