. 2006 Aug;50(8):2772–2781. doi: 10.1128/AAC.00127-06

TABLE 2.

Changes in EC₅₀ values (relative to wt EC₅₀) of VRX-413638, AZT, and FTC against NRTI-resistant HIV-1 isolates

NRTI resistance mutation(s)^a	Mean change (fold) in EC₅₀ ± SD^b
NRTI resistance mutation(s)^a	VRX-413638 (wt EC₅₀ = 27.1 ± 3.40 nM)	AZT (wt EC₅₀ = 16.4 ± 2.80 nM)	FTC (wt EC₅₀ = 42.5 ± 3.70 nM)
K65R	0.2 ± 0.03	0.6 ± 0.06	102.7 ± 7.16*
M41L, D67N, T69N, K70R, T215F, K219E	0.7 ± 0.14	21.0 ± 7.66*	13.5 ± 6.60*
M41L, E44D, D67N, T69D, V118I, L210W, T215Y	0.7 ± 0.19	389.1 ± 143.78*	25.8 ± 21.24*
V75I, F77L, F116Y, Q151M	0.9 ± 0.08	43.9 ± 9.43*	17.9 ± 6.99*
M184V	3.0 ± 0.66*	0.3 ± 0.03	Max*
M41L, K70R, T215F, K219E	1.4 ± 0.03	83.7 ± 21.37*	9.4 ± 4.30*
K65R, K70R, V75I, F77L, F116Y, Q151M	1.6 ± 0.65	Max*	Max*
D67N, T69N, K70R, M184V, T215F, K219Q	1.9 ± 0.01	4.8 ± 2.20*	Max*
M41L, D67N, V118I, M184V, L210W, T215Y	4.4 ± 1.18*	9.5 ± 0.91*	Max*
M41L, D67N, M184V, L210W, T215Y	5.7 ± 1.05*	13.2 ± 7.32*	Max*
M41L, E44D, D67N, T69D, V118I, M184V, L210W, T215Y	11.2 ± 0.87*	54.7 ± 8.68*	Max*
T69K, K70G, V75I, F77L, F116Y, Q151M, M184V	15.7 ± 2.98*	274.3 ± 33.35*	Max*

Molecular clones with K65R and M184V mutations were in the HIV-1 NL4-3 format. All other viruses were in pNLPFB and were obtained from the U.S. NIH AIDS Research and Reference Reagent Repository.

EC₅₀ values were determined using the reporter cell line HeLa-JC-53-LTR-Luci. Mean EC₅₀ values ± SD were from two tests. Max; the change could not be determined because the EC₅₀ was >10 μM; *, EC₅₀ was statistically significantly higher than that against wild-type virus (P < 0.05).