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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of 24-SMT expression in Escherichia coli BL21(DE3)/pET28TbSMT cells. Lane 1, 25 μg of soluble extract of cells prior to induction with IPTG; lane 2, 25 μg of soluble extract after 4 h of induction with 1 mM IPTG at 25°C for 4 h; lane 3, 25 μg of the insoluble fraction of cells after 4 h of induction with 1 mM IPTG at 25°C for 4 h.
