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Sensitivity of the PCR assay with DNA from S. schenckii. (A) First-round PCR assay. (B) Nested PCR assay. Lanes: M, molecular size marker (100-bp ladder [Promega]); 1, distilled water; 2, 4 × 10−11 g of S. schenckii DNA; 3, 4 × 10−12 g of S. schenckii DNA; 4, 4 × 10−13 g of S. schenckii DNA; 5, 4 × 10−14 g of S. schenckii DNA; 6, 4 × 10−15 g of S. schenckii DNA; 7, 4 × 10−16 g of S. schenckii DNA.
