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Detection of S. schenckii in DNA extracts of 12 clinical samples by nested PCR assay. Lanes: M, molecular size marker (100-bp ladder [Promega]); 1, positive control of S. schenckii; 2, distilled water; 3 to 14, patient no. 1 to 12, respectively. Lane 8 (patient no. 6) showed a negative result.
