TABLE 2.

Detection of S. schenckii in clinical samples by nested PCR assay

Patient no.	Resulta by:			Clinical presentation (location)
	Culture	PAS stain	Nested PCR
1	+	+	+	Sporotrichoid nodules (right arm)
2	+	+	+	Ulcerated nodules (neck)
3	+	−	+	Ulcerated nodules (right hand)
4	+	−	+	Sporotrichoid nodules (left arm)
5	−	+	+	Verrucous plaques (nose)
6	+	−	−	Ulcerated nodules (left hand)
7	+	−	+	Indurated papules (right hand)
8	+	−	+	Sporotrichoid nodules (right arm)
9	−	+	+	Verrucous plaques (face)
10	−	+	+	Verrucous plaques (left foot)
11	+	+	+	Indurated papulo plaques (right hand)
12	−	+	+	Sporotrichoid nodules (left arm)

^a+, positive; −, negative.