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. 2006 Aug;50(8):2782–2788. doi: 10.1128/AAC.00032-06

FIG. 2.

FIG. 2.

PCR-based excision in strain COL. (A) PCR-based detection of the chromosomal junction when SCCmec is excised (attB) using primers cL1 and cR2 (22). (B) PCR-based detection of the excised circular form of SCCmec (attSCC) using primers colA and colB. These PCRs were performed using genomic DNA obtained after strains containing ccrAB constructs were grown for 24 h as a template. (C) PCR-based detection of the chromosomal junction. (D) PCR-based detection of the excised circular form of SCCmec using genomic DNA isolated after the strains containing ccrAB constructs were grown for 48 h. Labels represent the strains from which ccrAB originated.