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Time-kill assays. MICs for daptomycin (D) were 2 μg/ml for C. difficile RMA 9396 (top) and C. perfringens RMA 10436 (middle) and 8 μg/ml for C. perfringens RMA 10649 (bottom). MICs for vancomycin (V) were 0.5 μg/ml for all three strains. Test concentrations for daptomycin and vancomycin were two, four, and eight times the MIC. The detection level is ≥20 CFU/ml.
