FIG. 4.
(Left panel) Yields of recombinant proteins obtained after cultivation of the production clones in 70-ml microfermentors with HDM medium. (Right panel) Ratios of the yields of recombinant proteins in 70-ml microfermentors containing HDM medium to the yields of recombinant proteins in Fernbach flasks containing 1 liter of modified Lennox medium. Analytical purification and quantification of His6-tagged soluble recombinant proteins were performed as described in Materials and Methods. Cultivation protocols, including temperature and timing of IPTG addition, were adapted to the systems, and the best production in each system was used to calculate the ratios. The genes encoding the proteins tested are indicated between the panels. Designations beginning with Rv and ML are designations for the reading frames of the M. tuberculosis and M. leprae genomes, respectively, whose descriptions are available at the websites http://genolist.pasteur.fr/TubercuList/ and http://genolist.pasteur.fr/Leproma/. The following other proteins were included: Trypanosoma cruzi proline racemase (TcPRAC), tobacco etch virus protease fused to maltose-binding protein (MBP-TEV), and Vga(A) of Staphylococcus aureus (VGA).