TABLE 1.

Microscopic and molecular assessment of the effectiveness of scraping C. parvum (Moredun isolate C1/03) oocysts from seeded samples deposited onto welled slides

No. of DAPI+ oocystsa (no. of empty oocysts)	N18SDIAG PCR resultb
2; 2 (2)	+; +
3; 3 (1)	+; +
4; 4	−; +
5; 5; 5; 5 (1)	+; +; +; +
6; 6; 6; 6; 6 (1); 6; 6; 6	+; +; +; +; +; +; −; +
7 (2)	+
8 (3); 8 (1)	−; +
9 (1); 9 (2); 9 (1); 9 (2)	+; +; +; +
10; 10 (1); 10 (2); 10	+; +; +; +
11; 11 (1); 11 (1); 11 (2); 11 (1)	+; +; +; +; +
12 (1); 12; 12 (2)	+; −; +
13; 13; 13; 13 (2)	+; +; +; +; +
15 (1); 15; 15	+; +; +
16	+
18; 18 (2); 18	−; +; +

^aFollowing reexamination of slides after scraping, only two slides (containing 18 oocysts each) retained oocysts (one each). Coverslips from four slides (containing 18, 11, 11, and 12 DAPI-positive oocysts) were examined microscopically after their removal and found to contain no oocysts.

^bSlides containing the same number of oocysts are presented in the same row and are separated by semicolons. Each PCR result separated by a semicolon represents the analysis of the slide shown in the first column.