FIG. 2.

InsTipα insertion sites and expression of the resulting InsTip1 fusion proteins. (A to E) Results for the five strains with InsTipα insertions in (A) atpD, (B) ppiD, (C) yraI, (D) yicO, and (E) yfdX. Top panels, schematic depiction of the insertion site and orientation of InsTipα into each target gene. The respective gene is represented by a black arrow and its designation, and its total length is indicated in bp. InsTipα is drawn schematically above the open reading frame, and its insertion site is indicated by a dotted line with the exact position of the nucleotide preceding the inserted sequence. Bottom panels, growth curves (filled squares) and β-Gal activities (filled bars) of WH207(λtet50)/pWH1911 and the respective candidate strains (open squares and open bars) grown at 37°C in MacConkey medium without indicator. Growth is expressed by the optical density at 600 nm (OD₆₀₀) on the left y axis. The right y axis displays the β-Gal activities in Miller units (MU) (23). Each value was determined in triplicate, and the standard deviations are shown (error bars).