FIG. 3.

Carbon source-dependent differential expression of PpiD-InsTip1. β-Gal activities of WH207(λtet50)/pWH1911 (closed bars) and WH852 (open bars) grown at 37°C to an optical density at 600 nm of 0.2 in M9 minimal medium supplemented with 50 mM glycerol or pyruvate. The y axis displays the β-Gal activities in Miller units (MU) (23). Each value was determined in triplicate and is shown with standard deviations (error bars). The dotted lines indicate the background β-Gal activities determined for the untagged parent strain, which were subtracted from the β-Gal activities of the tagged strain to determine the induction factor.