TABLE 1.
Bacterial strains, plasmids, and phage used in this study
| Strain, plasmid, or phage | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| RB791 | IN[rrnD-rrnE]1 lacIq PlacL8; cloning and propagation of pWH1909 and pWH2101 | 3 |
| WH207 | galK rpsL thi ΔlacX74 recA13 | 35 |
| WH851 | WH207(λtet50)/pWH1911 atpD1147::InsTipα; Kmr | This study |
| WH852 | WH207(λtet50)/pWH1911 ppiD328::InsTipα; Kmr | This study |
| WH853 | WH207(λtet50)/pWH1911 yraI607::InsTipα; Kmr | This study |
| WH854 | WH207(λtet50)/pWH1911 yicO850::InsTipα; Kmr | This study |
| WH855 | WH207(λtet50)/pWH1911 yfdX475::InsTipα; Kmr | This study |
| Plasmids and phage | ||
| pWH1866 | Apr Kmr; InsTetG−1; pUC18 derivative | 17 |
| pWH1909 | Apr KmrPtac TrxA-InsTip1; pBR322 derivative | This study |
| pWH1911 | CmrlacIqtetR; pACYC177 derivative | This study |
| pWH1912 | Apr Kmr; InsTipα; pUC18 derivative | This study |
| pWH2101 | AprPtac trxA-tip; pBR322 derivative | 16 |
| λtet50 | Tn10 tetA-lacZ transcriptional fusion | 32 |