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A sample precision plate. During the initial phase of growth, nonmutants rapidly grow to their maximum size, which is tiny. The small colonies are visible in the background of the inset. Mutations which restore function of PcaG allow growth with quinate and produce the large colonies visible in both pictures. The small colonies cease growth after 2 to 3 days; identifiable mutant colonies appear after 3 to 4 days and reach a readily distinguishable size by 5 to 6 days.
