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DNA methylation in E. coli. The E. coli MC1061 transformant with pMW1 was cultivated in 1% glucose or 0.02 or 0.2% arabinose, and the purified plasmid pMW1 was cleaved by RsaI; complete cleavage into fragments of the expected sizes was achieved (1% agarose gel visualized under UV light after ethidium bromide staining).
