TABLE 2.
Primers used in this studya
| Marker locus | Primer designation | Primer sequence |
|---|---|---|
| clfA | ClfA-F | 5′-GATTCTGACCCAGGTTCAGA |
| ClfA-R | 5′-CTGTATCTGGTAATGGTTCTTT | |
| clfB | ClfB-F | 5′-ATGGTGATTCAGCAGTAAATCC |
| ClfB-R | 5′-CATTATTTGGTGGTGTAACTCTT | |
| sdr | SdrCDE-F | 5′-GTAACAATTACGGATCATGATG |
| SdrCDE-R | 5′-TACCTGTTTCTGGTAATGCTTT | |
| spa | Spa-F | 5′-AGCACCAAAAGAGGAAGACAA |
| Spa-R | 5′-GTTTAACGACATGTACTCCGT | |
| ssp | SspA-F | 5′-ATCMATTTYGCMAAYGATGACCA |
| SspA-R | 5′-TTGTCTGAATTATTGTTATCGCC |
a
The primers were designed based on the completed and unfinished S. aureus genomes available from The Institute for Genomic Research (http://www.tigr.org) (COL strain), the University of Oklahoma (http://www.genome.ou.edu) (strain 8325), Sanger Institute (http://www.sanger.ac.uk) (strains MRSA252 and MSSA476), and Juntendo University (http://www.juntendo.ac.jp) (strains Mu50 and N315).