FIG. 5.
Northern analysis of OnBt-R1 expression in Cry1Ab-susceptible and -resistant O. nubilalis strains. A positive nylon membrane containing total RNA (7.5 μg/lane) was probed with DIG-labeled oligonucleotide corresponding to the OnBt-R1 gene fragment. Lanes 1, 2, and 3 represent Europe-S (E-S), Europe-R (E-R), and RSTT-R (R-T), respectively. (A) Cadherin-probe hybridization to a 5.2-kb mRNA band. (B) The membrane was stripped and reprobed with the DIG-RPS3 probe for normalization, with hybridization to a 0.8-kb mRNA band. Mr DIG-labeled RNA marker II (Roche Applied Science, Indianapolis, IN).