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. 2006 Aug;72(8):5670–5672. doi: 10.1128/AEM.00609-06

FIG. 4.

FIG. 4.

Cassette for integration of STS and 4CL into the yeast genome. LEU21-546, LEU2 gene from nucleotides 1 to 546 of the coding region; Pi, promoter of the ILV2 gene; ILV2-SMR, sulfometuron methyl resistance gene; Ti, terminator of the ILV2 gene; Tc, terminator of the CYC gene; STS, coding region of the grape STS gene; PGAL1, promoter of the GAL1 gene; PGAL10, promoter of the GAL10 gene; 4CL, coding region of the 4CL2 gene from tobacco; Ta, terminator of the ADH1 gene; LEU2547-1060, LEU2 gene from nucleotides 547 to 1060 of the coding region; PacI, unique PacI restriction site.