TABLE 1.
Genomic identification and description of qRT-PCR targets
| Locus taga | Gene or gene product description | Protein designationb | Gene category |
|---|---|---|---|
| 0603 | DNA-dependent RNA polymerase, beta subunit | RpoB | Housekeeper |
| 0187 | Formate dehydrogenase, alpha subunit, putative | Fdh | Other respiration-associated |
| 0926 | Proton-translocating NADH-quinone oxidoreductase, D subunit, putative | Nuo | |
| 0562 | ATP synthase, F1 alpha subunit | AtpA | |
| 0103 | Molybdopterin oxidoreductase, iron-sulfur binding subunit, putative | Mod | |
| 0110 | [Ni/Fe] hydrogenase, group 1, large subunit, putative | Hup | Hydrogenase |
| 0615 | Hydrogenase, group 3, VhuA subunit, putative | Vhu | |
| 0867 | Hydrogenase, group 4, EchE subunit, putative | Ech | |
| 1571 | Hydrogenase, group 4, HycE subunit, putative | Hyc | |
| 0147 | [Fe] hydrogenase, large subunit HymC, putative | Hym | |
| 0079 | Trichloroethene reductive dehalogenase (tceA) gene | TceA | Reductive dehalogenase |
| 0318 | Reductive dehalogenase, putative | PceAc | |
| 1545 | Reductive dehalogenase, putative | ||
| 0162 | Reductive dehalogenase, putative, auth pt mutationd | ||
| 1559 | Reductive dehalogenase, putative | ||
| 0173 | Reductive dehalogenase, putative | ||
| 0876 | Reductive dehalogenase, putative | ||
| 0306 | Reductive dehalogenase, putative | ||
| 1519 | Reductive dehalogenase, putative |
a
Open reading frame identification number from the D. ethenogenes strain 195 genome.
b
Designation of known or putative protein.
c
S. H. Zinder, personal communication.
d
auth pt mutation, authentic point mutation.