TABLE 1.
Amplified sequences of A. marginale isolates from Oklahoma and their characteristicsa
| Isolate | Characteristics (location, host clinical parameters, date) | Genes (no. of repeats for MSP1a) | GenBank accession no. |
|---|---|---|---|
| Oklahoma | Wetumka, Hughes Co., 1997 | msp1α (3) | AY010247 |
| MSP4 | AY010252 | ||
| Glencoe 1 | Hesser Dairy; Holstein-Friesian herd; cow 23; Glencoe, Payne Co.; PCV = 15%; infection = 2.4%; Aug. 2001 | msp1α (5) | AY127053 |
| msp4 | AY127067 | ||
| Glencoe 2 | Hesser Dairy; Holstein-Friesian herd; cow 14; Glencoe, Payne Co.; PCV = 15%; infection = 1.4%; Sept. 2001 | msp1α (4) | AY127054 |
| msp4 | AY127068 | ||
| Glencoe 3 | Hesser Dairy; Holstein-Friesian herd; cow 242; Glencoe, Payne Co.; PCV = 18.5%; infection = 4.9%; Nov. 2001 | msp1α (3) | AY127055 |
| msp4 | AY127069 | ||
| Cushing 1 | Kuykendall Dairy; Aberdeen Angus herd; Cow “Dusty”; Cushing, Payne Co. PCV = 12.7%; infection = 59.9%; Sept. 2001 | msp1α (4) | AY127056 |
| msp4 | AY127070 | ||
| Cushing 2 | Kuykendall Dairy; Aberdeen Angus herd; cow “Rassy”; Cushing, Payne Co.; PCV = 35%; infection = 0.1%; Nov. 2001 | msp1α (5) | AY127057 |
| msp4 | AY127071 | ||
| Stigler | Jones Dairy; Stigler, Haskell Co; PCV = 10%; infection = 48%; Oct. 2001 | msp1α (4) | AY127058 |
| msp4 | AY127072 | ||
| Oklahoma City | Oklahoma City Market (now in Newalla, Oklahoma Co); PCV = 8.5%, infection = 9.8%; Oct. 2001 | msp1α (1) | AY127059 |
| msp4 | AY127073 | ||
| Okmulgee | Okmulgee, Okmulgee Co.; PCV = 11.1%, infection = 49.5%; Oct. 2001 | msp1α (4) | AY127060 |
| msp4 | AY127074 | ||
| Stillwater 1 | Myron dairy; cow 1; 823 N. Bethel, Stillwater, Payne Co.; PCV = 30%; infection = 0.3%; Aug. 2001 | msp1α (5) | AY127061 |
| msp4 | AY127075 | ||
| Stillwater 2 | Myron dairy; cow 2; 823 N. Bethel, Stillwater, Payne Co.; PCV = 18.2%; infection = 3.7%; Oct. 2001 | msp1α (4) | AY127062 |
| msp4 | AY127076 | ||
| New Castle | New Castle, McClain Co.; PCV = 10.5%; infection = 6.3%; Oct. 2001 | msp1α (4) | AY127063 |
| msp4 | AY127077 | ||
| Pawhuska | Pawhuska, Osage Co., 1960s | msp1α (2) | AY127064 |
| msp4 | AY127078 |
a
All isolates were obtained from naturally infected cattle. Isolates Oklahoma and Pawhuska were originally obtained from naturally infected cattle and for this study were isolated from bovine erythrocytes collected from experimentally infected cattle. Whole blood was collected in VACUTAINER tubes containing EDTA and used for preparation of stained blood smears for counting of percent infected erythrocytes (infection) by light microscopy and for determination of the packed cell volume (PCV).