TABLE 2.
Bacterial strains used in this study to test specificity of the nosZ1 and nosZ2 primers
| Strain | Group | Presence of nosZ | N2O reduction | Quantitative PCR result with primer type:
|
|
|---|---|---|---|---|---|
| nosZ1 | nosZ2 | ||||
| Bacillus senegalensis M1518 | Firmicutes | NDb | + | − | − |
| Bacillus cereus M944 | Firmicutes | ND | + | − | − |
| Bacillus licheniformis SN530 | Firmicutes | ND | − | − | − |
| Bradyrhizobium japonicum 562 | α-Proteoa | + | ND | + | − |
| Bradyrhizobium japonicum USDA110 | α-Proteo | + | ND | + | + |
| Sinorhizobium morelense SN611 | α-Proteo | + | ND | + | + |
| Hyphomicrobium denitrificans DSM 1869 | α-Proteo | + | ND | + | − |
| Sinorhizobium meliloti 1021 | α-Proteo | + | ND | + | + |
| Rhizobium meliloti 50 | α-Proteo | + | ND | + | + |
| Alcaligenes faecalis ATCC 8750 | β-Proteo | + | ND | + | + |
| Achromobacter cycloclastes ATCC 21921 | β-Proteo | + | ND | + | + |
| Achromobacter xylosoxidans M129 | β-Proteo | + | + | + | + |
| Pseudomonas denitrificans CCUG 2519 | γ-Proteo | + | ND | + | − |
| Pseudomonas fluorescens C7R12 | γ-Proteo | + | + | + | + |
| Escherichia coli JM109 | γ-Proteo | − | − | − | − |
a
Proteo, proteobacteria.
b
ND, not determined.