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Proposed partial pathway for biodegradation of 1,4-dioxane by strain ENV478. The 1,4-dioxane-2-ol (hemiacetal) and 2-hydroxyethoxy-2-acetaldehyde were not detectable in the culture media by the methods used in this study. PDX is expected to form spontaneously from 2HEAA under certain conditions (42), but it was not detectable in our experiments.
