TABLE 4.
Expression of pcaF-gusA fusion in S. meliloti wild-type and PcaR− backgrounds
| Strain | Relevant genotype | Growth condition | β-Glucuronidase activitya (Miller units [SD]) |
|---|---|---|---|
| RmK1015 | Rm1021 pcaF::gusA | Glycerol | 27 (2.7) |
| Glycerol plus adipate | 131 (11.7) | ||
| Glycerol plus PCAb | 259 (18.6) | ||
| RmK1016 | Rm1021 pcaF::gusA pcaR::Ω | Glycerol | 52 (3.0) |
| Glycerol plus adipate | 48 (4.1) | ||
| Glycerol plus PCA | 66 (6.8) |
a
Shown are averages of values obtained from three independent cultures grown overnight in LBmc and subcultured into M9 minimal medium with 0.5% glycerol ± 5 mM protocatechuate or 30 mM adipate at 30°C for 4 h.
b
PCA, protocatechuate.