TABLE 5.
Expression of pcaR-gusA fusion in S. meliloti wild-type and PcaR− backgrounds
| Strain | Relevant genotype | Growth condition | β-Glucuronidase activitya (Miller units [SD]) |
|---|---|---|---|
| RmP892 | Rm1021 (pTH1335) | Glycerol | 62 (2.2) |
| Glycerol plus PCAb | 42 (2.6) | ||
| RmP893 | Rm1021 pcaR::Ω (pTH1335) | Glycerol | 291 (8.2) |
| Glycerol plus PCA | 261 (3.1) |
a
Shown are averages of values obtained from three independent cultures grown overnight in LBmc and subcultured into M9 minimal medium with 0.5% glycerol ± 5 mM protocatechuate at 30°C for 4 h.
b
PCA, protocatechuate.