TABLE 1.
Results of microarray analysis showing transcripts significantly up- or down-regulated in calcifying phosphate-limited relative to noncalcifying phosphate-replete E. huxleyi 1516 culturesa
| Gene identification no. | Homolog description | E value | P value | FCb |
|---|---|---|---|---|
| 77-2-5-E02.r.5 | No hit | 3.00E−04 | 29.19 | |
| 77-2-35-G03.r.1 | No hit | 1.09E−06 | 27.99 | |
| 77-2-9-C04.r.1 | No hit | 2.58E−08 | 21.52 | |
| Contig 22 | Hypothetical protein | 4.82E−24 | 3.21E−07 | 20.60 |
| 77-2-41-G02.r.1 | No hit | 8.63E−07 | 18.49 | |
| 77-2-8-C07.r.1 | No hit | 2.46E−07 | 18.48 | |
| 77-2-36-H06.r.1 | No hit | 2.07E−03 | 13.53 | |
| 77-2-36-H03.r.2 | No hit | 9.05E−06 | 13.10 | |
| 77-2-9-E03.r.5 | No hit | 1.58E−07 | 13.05 | |
| Contig 15 | Hypothetical protein | 4.95E−03 | 1.80E−08 | 12.07 |
| 77-2-6-H01.r.2 | No hit | 3.63E−07 | 11.18 | |
| 77-2-32-E05.r.1 | No hit | 1.26E−07 | 10.85 | |
| Contig 9 | No hit | 2.11E−06 | 10.43 | |
| 77-2-41-F03.r.1 | Hypothetical protein | 3.26E−03 | 1.70E−06 | 9.93 |
| Contig 24 | Alpha-soluble NSF attachment protein | 6.91E−23 | 2.87E−07 | 9.51 |
| 77-2-4-E06.r.1 | RabGAP/TBC domain-containing protein | 9.61E−22 | 1.22E−03 | 9.27 |
| Contig 20 | No hit | 3.10E−06 | 9.06 | |
| 77-2-4-G05.r.1 | No hit | 2.38E−09 | 8.85 | |
| Contig 18 | No hit | 2.46E−05 | 8.52 | |
| 77-2-31-F12.r.1 | No hit | 2.62E−07 | 7.70 | |
| Contig 14 | No hit | 2.38E−06 | 7.54 | |
| 77-2-5-B05.r.2 | No hit | 9.21E−05 | 7.36 | |
| 77-2-39-G04.r.1 | No hit | 4.51E−05 | 6.84 | |
| 77-2-8-G04.r.1 | No hit | 6.38E−09 | 6.74 | |
| 77-2-41-E04.r.1 | No hit | 1.68E−05 | 6.43 | |
| 77-2-9-C12.r.1 | Surface antigen BspA, Bacteroides forsythus | 9.03E−37 | 4.99E−05 | 6.23 |
| 77-2-39-E03.r.1 | Predicted dinucleotide-binding enzymes | 7.34E−05 | 7.03E−05 | 5.96 |
| 77-2-8-A07.r.1 | LigA | 2.31E−56 | 1.78E−09 | 5.57 |
| 77-2-15-F10.r.2 | No hit | 1.63E−09 | 5.54 | |
| 77-2-12-C02.r.1 | Guanylate cyclase | 6.27E−08 | 4.98E−05 | 5.44 |
| 77-2-40-B09.r.1 | No hit | 3.14E−06 | 5.39 | |
| Contig 10 | Predicted esterase | 1.83E−05 | 7.11E−06 | 5.03 |
| 77-2-36-F03.r.2 | l-3-Phosphoserine phosphatase | 3.80E−50 | 1.23E−06 | 5.0 |
| Contig 21 | No hit | 7.53E−04 | 4.91 | |
| 77-2-11-H03.r.2 | No hit | 1.24E−06 | 4.88 | |
| Contig 12 | No hit | 9.41E−06 | 4.77 | |
| 77-2-8-C12.r.1 | Outer mitochondrial membrane protein porin | 4.21E−22 | 1.65E−08 | 4.65 |
| 77-2-7-D01.r.1 | No hit | 2.43E−05 | 4.59 | |
| Contig 19 | No hit | 2.42E−06 | 4.38 | |
| 77-2-38-B11.r.1 | No hit | 2.05E−07 | 4.25 | |
| Contig 16 | No hit | 4.93E−05 | 4.23 | |
| 77-2-8-C05.r.1 | No hit | 3.82E−05 | 4.19 | |
| Contig 6 | Hypothetical protein | 2.99E−30 | 1.87E−05 | 4.03 |
| Contig 7 | No hit | 2.55E−04 | 3.98 | |
| 77-2-16-D04.r.1 | No hit | 1.84E−07 | 3.76 | |
| 77-2-4-B06.r.1 | Possible elongation of very-long-chain fatty acid protein | 3.44E−19 | 5.72E−05 | 3.76 |
| 77-2-15-E10.r.2 | No hit | 1.78E−05 | 3.47 | |
| 77-2-9-D02.r.5 | Putative serine/threonine kinase | 1.38E−39 | 4.28E−06 | 3.30 |
| 77-2-41-E05.r.1 | No hit | 5.55E−07 | 3.14 | |
| 77-2-7-A10.r.5 | IMP dehydrogenase/GMP reductase | 4.03E−10 | 3.56E−05 | 3.13 |
| 77-2-8-E04.r.1 | No hit | 4.47E−06 | 3.0 | |
| Contig 3 | No hit | 3.15E−07 | 2.97 | |
| 77-2-24-F03.r.1 | Cytochrome b5 domain-containing protein-like | 8.30E−14 | 3.57E−08 | 2.9 |
| 77-2-19-E12.r.1 | Small nuclear ribonucleoprotein polypeptide D2 | 2.47E−35 | 1.40E−08 | 2.74 |
| 77-2-34-B09.r.1 | No hit | 4.98E−07 | 2.61 | |
| 77-2-14-G05.r.1 | No hit | 3.98E−05 | 2.60 | |
| 77-2-24-H06.r.1 | No hit | 6.53E−09 | 2.60 | |
| 77-2-35-H12.r.1 | Hypothetical protein | 2.17E−03 | 8.76E−05 | 2.60 |
| 77-2-7-B09.r.4 | Proton/amino acid transporter 2 | 5.19E−09 | 4.40E−04 | 2.57 |
| 77-2-9-C08.r.3 | Expressed protein | 7.82E−15 | 8.60E−05 | 2.53 |
| 77-2-18-E09.r.1 | Asparaginyl endopeptidase | 7.83E−70 | 1.94E−05 | 2.49 |
| 77-2-19-F06.r.1 | Phosphate-repressible phosphate permease | 8.44E−04 | 4.22E−04 | 2.41 |
| 77-2-8-B04.r.1 | 4 Fe-S ferridoxin | 1.19E−03 | 2.70E−04 | 2.40 |
| 77-2-14-C05.r.1 | No hit | 8.24E−05 | 2.39 | |
| Contig 13 | No hit | 1.18E−04 | 2.33 | |
| 77-2-38-H09.r.4 | No hit | 2.08E−06 | 2.33 | |
| 77-2-7-A09.r.1 | Putative ammonium transporter | 2.55E−53 | 6.02E−04 | 2.28 |
| 77-2-41-E09.r.1 | Arachidonate 15-lipoxygenase, second type | 5.34E−05 | 4.29E−03 | 2.26 |
| 77-2-40-E03.r.1 | No hit | 2.14E−06 | 2.22 | |
| 77-2-7-H02.r.2 | 4-Aminobutyrate aminotransferase | 9.05E−103 | 1.45E−04 | 2.13 |
| 77-2-19-C01.r.1 | Putative gamma-carbonic anhydrase | 1.73E−48 | 3.31E−05 | 2.10 |
| 77-2-16-F04.r.1 | Hypothetical protein | 8.29E−14 | 4.17E−04 | 2.09 |
| 77-2-1-A06.r.1 | Hypothetical protein | 8.30E−13 | 4.00E−04 | 2.06 |
| 77-2-7-B11.r.5 | Hypothetical protein | 1.85E−14 | 2.73E−04 | 2.06 |
| 77-2-35-D05.r.1 | No hit | 3.70E−04 | 2.05 | |
| Contig 17 | No hit | 2.10E−04 | 2.04 | |
| 77-2-7-G03.r.2 | Similar to ankyrin 2 | 1.44E−18 | 1.38E−03 | 2.04 |
| 77-2-30-H04.r.1 | Aspartate aminotransferase | 5.02E−95 | 6.91E−05 | 2.02 |
| 77-2-15-F09.r.2 | No hit | 2.46E−04 | 2.01 | |
| 77-2-11-H04.r.3 | No hit | 4.24E−04 | −2.02 | |
| 77-2-11-G02.r.1 | No hit | 4.05E−03 | −2.03 | |
| 77-2-11-A09.r.1 | No hit | 1.04E−04 | −2.05 | |
| 77-2-11-A05.r.1 | No hit | 9.82E−03 | −2.07 | |
| 77-2-19-H01.r.1 | N-Acetylneuraminic acid phosphate synthase | 3.92E−17 | 5.36E−04 | −2.11 |
| 77-2-33-C08.r.1 | Hypothetical protein | 1.59E−03 | 3.28E−05 | −2.13 |
| 77-2-11-C12.r.5 | No hit | 2.39E−04 | −2.14 | |
| 77-2-19-B04.r.1 | Conserved hypothetical protein | 7.24E−09 | 7.55E−07 | −2.20 |
| 77-2-10-C04.r.2 | No hit | 3.64E−05 | −2.26 | |
| 77-2-19-G10.r.1 | No hit | 6.05E−04 | −2.27 | |
| Contig 5 | Fucoxanthin-chlorophyll a to c binding protein F | 6.58E−09 | 1.02E−03 | −2.31 |
| 77-2-11-A12.r.1 | No hit | 2.39E−04 | −2.45 | |
| 77-2-10-D09.r.3 | No hit | 2.85E−05 | −2.57 | |
| 77-2-14-F02.r.1 | No hit | 5.35E−07 | −2.64 | |
| 77-2-14-B10.r.1 | No hit | 2.78E−06 | −2.72 | |
| 77-2-16-A09.r.1 | No hit | 1.20E−03 | −2.74 | |
| 77-2-12-F03.r.2 | Proteophosphoglycan | 2.35E−03 | 2.19E−03 | −2.80 |
| 77-2-5-F11.r.4 | Proteasome alpha subunit | 4.13E−77 | 3.90E−06 | −2.84 |
| 77-2-5-H09.r.3 | No hit | 1.16E−03 | −2.87 | |
| 77-2-33-D03.r.1 | No hit | 1.76E−05 | −2.91 | |
| 77-2-24-F09.r.1 | No hit | 3.73E−03 | −2.97 | |
| Contig 23 | Ac1147 | 4.34E−25 | 5.13E−05 | −3.04 |
| 77-2-30-E06.r.1 | No hit | 2.28E−03 | −3.32 | |
| 77-2-38-F06.r.1 | No hit | 7.67E−05 | −3.40 | |
| 77-2-39-H02.r.2 | GA12046-PA | 7.51E−13 | 9.79E−04 | −3.57 |
| 77-2-3-B03.r.2 | Cysteine protease | 1.01E−60 | 4.77E−04 | −3.82 |
| 77-2-30-E07.r.1 | Hypothetical protein | 2.12E−149 | 3.79E−03 | −3.83 |
| 77-2-12-D03.r.1 | Novel protein | 2.00E−10 | 1.24E−03 | −3.93 |
| 77-2-30-G06.r.1 | Phosphoglycerate/bisphosphoglycerate mutase family protein | 4.60E−18 | 7.24E−04 | −3.94 |
| 77-2-12-E01.r.2 | No hit | 5.87E−03 | −3.94 | |
| 77-2-24-E01.r.1 | No hit | 5.20E−04 | −4.03 | |
| 77-2-4-H01.r.2 | No hit | 4.71E−05 | −4.30 | |
| Contig 4 | Fucoxanthin chlorophyll a/c protein | 5.65E−24 | 5.90E−08 | −4.34 |
| 77-2-12-F04.r.1 | Predicted permeases | 2.93E−39 | 7.18E−03 | −4.43 |
| Contig 25 | No hit | 2.12E−05 | −4.58 | |
| 77-2-12-B04.r.1 | No hit | 3.34E−03 | −4.59 | |
| 77-2-24-D02.r.1 | Sulfatase 1 precursor | 8.29E−71 | 8.16E−04 | −4.66 |
| 77-2-24-H08.r.1 | Peptidyl-prolyl cis-trans-isomerase | 1.23E−19 | 4.09E−03 | −4.80 |
| 77-2-24-E02.r.1 | No hit | 1.14E−04 | −4.80 | |
| 77-2-11-A10.r.5 | Trypsin | 5.78E−31 | 6.48E−04 | −4.82 |
| 77-2-31-F09.r.1 | No hit | 5.47E−07 | −5.23 | |
| 77-2-31-C11.r.1 | No hit | 1.92E−05 | −5.38 | |
| 77-2-29-H11.r.1 | No hit | 1.09E−06 | −5.76 | |
| 77-2-12-C05.r.1 | Proteophosphoglycan | 6.16E−04 | 6.84E−03 | −5.93 |
| 77-2-2-B09.r.1 | Similar to kinesin light chain | 1.93E−03 | −6.29 | |
| 77-2-19-E11.r.1 | No hit | 3.02E−04 | −6.32 | |
| 77-2-7-A05.r.1 | Unnamed protein product | 1.88E−12 | 9.02E−08 | −6.66 |
| 77-2-16-G05.r.1 | No hit | 2.08E−04 | −10.59 |
a
The results of a translated query to the NCBI peptide database are shown, including homologues with an E value of <10−2.
b
Relative gene expression is expressed as FC with the corresponding P value. For assembled contigs, a mean FC value is given.