TABLE 2.
Real-time RT-PCR validation of cDNA microarray relative gene expression
| Gene identification no.a | FC byb:
|
|
|---|---|---|
| Microarray | Real-time RT-PCR | |
| Contig 24 | 9.51 | 134.05 |
| Contig 20 | 9.06 | 53.20 |
| 77-2-19-F06.r.1 | 2.41 | 35.10 |
| Contig 18 | 8.52 | 34.30 |
| Contig 7 | 3.98 | 28.51 |
| 77-2-7-B09.r.4 | 2.57 | 18.38 |
| Contig 16 | 4.23 | 16.37 |
| Contig 21 | 4.91 | 9.40 |
| 77-2-7-D01.r.1 | 4.59 | 3.17 |
| 77-2-18-E09.r.1 | 2.49 | 3.17 |
| 77-2-7-G03.r.2 | 2.04 | 2.64 |
| 77-2-12-C02.r.1 | 5.44 | 2.05 |
| 77-2-12-E01.r.2 | −3.94 | 1.87* |
| Contig 5 | −2.31 | 1.74* |
| 77-2-41-E04.r.1 | 6.43 | 1.66 |
| 77-2-19-C01.r.1 | 2.10 | 1.66 |
| 77-2-19-E12.r.1 | 2.74 | 1.66 |
| 77-2-40-B09.r.1 | 5.39 | 1.02 |
| 77-2-12-F04.r.1 | −4.43 | −1.23* |
| 77-2-24-H08.r.1 | −4.80 | −3.03 |
a
Twenty transcripts up- and down-regulated in cDNA microarray randomly selected for real-time RT-PCR analysis.
b
Relative gene expression in phosphate-limiting relative to phosphate-replete E. huxleyi cultures expressed as FC. Asterisks indicate transcripts that were not validated by real-time RT-PCR.