TABLE 4.
Transcripts most likely to be involved in phosphate limitation and/or microarray false positivesa
| Gene identification no. | Homolog descriptionb | FC by:
|
|
|---|---|---|---|
| Microarray for 1516 P− vs 1516 P+ | Real-time RT-PCR for B39 P+ vs 1516 P+ | ||
| 77-2-24-D02.r.1 | Sulfatase 1 precursor | −4.66 | 106.40 |
| 77-2-11-H04.r.3 | No hit | −2.02 | 23.16 |
| 77-2-24-E02.r.1 | No hit | −4.80 | 18.38 |
| 77-2-11-C12.r.5 | No hit | −2.14 | 18.38 |
| 77-2-12-E01.r.2 | No hit | −3.94 | 10.08 |
| Contig 25 | No hit | −4.58 | 7.29 |
| 77-2-12-F03.r.2 | Proteophosphoglycan | −2.80 | 6.50 |
| 77-2-11-A12.r.1 | No hit | −2.45 | 2.83 |
| 77-2-30-E07.r.1 | EH domain protein | −3.83 | 1.87 |
| 77-2-19-G10.r.1 | No hit | −2.27 | 1.59 |
| Contig 5 | Fucoxanthin-chlorophyll A-to-C binding protein F | −2.31 | 1.52 |
| 77-2-14-C05.r.1 | No hit | 2.39 | 1.35 |
| 77-2-24-E01.r.1 | No hit | −4.03 | 1.18 |
| 77-2-11-A10.r.5 | Similar to transmembrane serine protease 9 | −4.82 | 1.15 |
| 77-2-11-H03.r.2 | No hit | 4.88 | 1.07 |
| 77-2-30-G06.r.1 | Phosphoglycerate/bisphosphoglycerate mutase | −3.94 | 1.02 |
| 77-2-12-C02.r.1 | Adenylate cyclase, family 3 | 5.44 | 1.00 |
| 77-2-24-H07.r.1 | Peptidyl-prolyl cis-trans-isomerase | −4.08 | −1.05 |
| 77-2-24-F09.r.1 | No hit | −2.97 | −1.08 |
| 77-2-7-B09.r.4 | Proton/amino acid transporter | 2.57 | −1.12 |
| 77-2-16-A09.r.1 | No hit | −2.74 | −1.12 |
| Contig 12 | No hit | 4.77 | −1.15 |
| 77-2-7-A09.r.1 | Putative ammonium transporter | 2.28 | −1.23 |
| 77-2-12-F04.r.1 | Predicted permeases | −4.43 | −1.23 |
| 77-2-33-C08.r.1 | hypothetical protein | −2.13 | −1.28 |
| 77-2-31-F12.r.1 | No hit | 7.70 | |
| 77-2-8-A07.r.1. | Putative calcium binding protein | 5.57 | −1.96 |
| 77-2-8-C12.r.1 | Outer mitochondrial membrane protein porin | 4.65 | −2.50 |
| 77-2-40-B09.r.1 | No hit | 5.39 | −2.56 |
| 77-2-16-D04.r.1 | No hit | 3.76 | −2.78 |
| 77-2-8-E04.r.1 | No hit | 3.00 | −5.26 |
| Contig 9 | No hit | 10.43 | −7.14 |
| 77-2-7-D01.r.1 | No hit | 4.59 | −14.29 |
| 77-2-18-E09.r.1 | Asparaginyl endopeptidase | 2.49 | −14.29 |
| 77-2-41-F03.r.1 | Hypothetical protein | 9.93 | −20.00 |
| 77-2-36-H06.r1 | No hit | 13.53 | −25.00 |
a
Shown are results for transcripts that exhibited differential expression in calcifying 1516 cultures relative to noncalcifying 1516 E. huxleyi cultures grown in phosphate-limited media (P−) relative to noncalcifying cultures of 1516 grown in phosphate-replete media (P+). These transcripts also showed no differential expression or discordant relative expression in calcifying B39 cultures compared to noncalcifying 1516 cultures when grown in identical phosphate-replete media as determined by real-time PCR analysis.
b
The results of a translated query to the NCBI peptide database are shown (E value of <10−2).