TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference |
|---|---|---|
| Strains | ||
| Rhodococcus sp. strain RHA1 | Wild type; BPH+ ETB+ | 17 |
| Rhodococcus sp. strain HDT1 | Mutant derivative of RHA1; aphII gene insertion mutant of etbA1; Kmr | This study |
| Rhodococcus sp. strain HDB1 | Mutant derivative of RHA1; aphII gene insertion mutant of ebdA1; Kmr | This study |
| Rhodococcus sp. strain HDA1 | Mutant derivative of RHA1; aphII gene insertion mutant of bphA1; Kmr | This study |
| Rhodococcus sp. strain HDB3 | Mutant derivative of RHA1; aphII gene insertion mutant of ebdA3; Kmr | This study |
| Rhodococcus sp. strain HDA3 | Mutant derivative of RHA1; aphII gene insertion mutant of bphA3; Kmr | This study |
| Rhodococcus sp. strain HDT4 | Mutant derivative of RHA1; aphII gene insertion mutant of etbA4; Kmr | This study |
| Rhodococcus sp. strain HDAT1 | Mutant derivative of HDA1; cmrA gene insertion mutant of etbA1; Kmr Cmr | This study |
| Rhodococcus sp. strain HDAB1 | Mutant derivative of HDA1; cmrA gene insertion mutant of ebdA1; Kmr Cmr | This study |
| Rhodococcus sp. strain HDBT1 | Mutant derivative of HDB1; cmrA gene insertion mutant of etbA1; Kmr Cmr | This study |
| Rhodococcus sp. strain HDAB33 | Mutant derivative of HDA3; cmrA gene insertion mutant of ebdA3; Kmr Cmr | This study |
| Escherichia coli JM109 | recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK−) e14 (mcrA) supE44 relA1 Δ(lac-proAB) [F′ traD36 proAB lacIqZΔM15] | |
| Plasmids | ||
| pBluescript II SK(+) | Cloning vector; Apr | Stratagene |
| pETQ1 | pUC119 with 10.0-kb HindIII fragment of RHA1 carrying etbA1A2C and bphD1 | This study |
| pHX11 | pUC119 with 3.8-kb XhoI fragment of RHA1 carrying ebdA1A2A3 | This study |
| pK4tsr | pK4 with insertion of tsr gene from pBsRG6; Kmr Tsr | This study |
| pBlueKm | pBluescript II SK(+) with insertion of aphII gene; Apr Kmr | This study |
| pBKE2 | pBlueKm containing a 761-bp StuI-PvuII etbA1 internal fragment | This study |
| pBAK1 | pBlueKm containing a 1,099-bp Eco52I bphA1 internal fragment | This study |
| pBKEA3 | pBlueKm containing a 330-bp AatII-NarI ebdA3 internal fragment | This study |
| pBKBA3 | pBlueKm containing a 302-bp PCR-amplified bphA3 internal fragment | This study |
| pBKA4-2 | pBlueKm containing a 731-bp MluI-SmaI etbA4 internal fragment | This study |
| pBlueCm | pBluescript II SK(+) with insertion of cmrA gene; Apr Cmr | This study |
| pBCEA1 | pBlueCm containing a 761-bp StuI-PvuII etbA1 internal fragment | This study |
| pBCEB3 | pBlueCm containing a 330-bp AatII-NarI ebdA3 internal fragment | This study |
| pK4TEB1 | pK4tsr with 2.3-kb BamHI-MluI fragment of RHA1 carrying ebdA1 | This study |
| pK4TEB3 | pK4tsr with 1.0-kb EcoRV-KpnI fragment of RHA1 carrying ebdA3 | This study |
| pK4TBA21 | pK4tsr with 2.7-kb EcoRI-NspV fragment of RHA1 carrying bphA1 | This study |
| pK4TBA3 | pK4tsr with 0.5-kb NspV-StuI fragment of RHA1 carrying bphA3 | This study |
| pK4TBA4-2 | pK4tsr with 2.0-kb BglII-HindIII fragment of RHA1 carrying etbA4 | This study |
a
BPH+, growth on biphenyl; ETB+, growth on ethylbenzene; Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Tsr, thiostrepton resistance.