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Examples of helper probes used for bead capture hybridization. The unlabeled upstream and downstream Bact338 helper probes (Table 1) were used at a 1:1:1 ratio with the biotin-labeled Bact338 capture probe. Note that the gill samples included both prokaryotic and eukaryotic rRNAs; Bathymodiolus azoricus gills harbor symbiotic bacteria (7).
