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(A) Examples of sediment-extracted total RNA. The RNA in each of the sample lanes represents approximately 7.5 mg of sediment, out of a total of approximately 30 g. The pure-culture reference RNAs were Saccharomyces cerevisiae RNA (S.c.) and Escherichia coli RNA (E.c.) (approximately 200 ng each). (B) Examples of magnetic bead capture hybridization with the Bact338 and Euk1379 probes. LSU, long subunit; euk., eukaryote; prok., prokaryote; LMW, low molecular weight.
