Figure 2.

EXT1 and EXT2 comigrate to the Golgi apparatus. Monolayers of BHK cells were transfected with EXT1-GFP (A), mEXT2-GFP (B), or both (C). When transfected into the same cell, EXT1-GFP and EXT2-GFP relocated to the Golgi (D), while the Golgi apparatus was immunolabeled with an anti-Golgi 58K monoclonal antibody and a Texas red-conjugated secondary antibody (E). When overlaid, they show excellent colocalization (yellow) (F). GFP fusion constructs of the EXT homologs EXTL2 (G) and EXTL3 (H) were also localized, as well as the murine N-deacetylase/N-sulfotransferase (NDST2), a key enzyme in HS biosynthesis (I).