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Mutant EXT1 proteins have different intracellular localizations. Monolayers of BHK cells were tranfected with 335-aa-EXT1-GFP (A), 335–EXT1-GFP and mEXT2-Myc-His (B–D), G339DEXT1-GFP (E), or G339DEXT1-Myc-His and mEXT2-GFP (F–H). After 30 h of expression, Myc-His-tagged EXT1 or EXT2 proteins were detected with an anti-His monoclonal antibody and a Texas red-conjugated secondary antibody.
