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PCR RAPD typing for 24 transconjugants (lanes 1 to 24) and recipient R (negated gel image). (a) RAPD1 with primer 1; (b) RAPD2 with primer 2; (c) RAPD3 with primer 3. Lane M, molecular size standard. In panels a and b, SPP1 DNA was cleaved with EcoRI; panel c is a mix of pBR322 cleaved with Bgl2 and HinfI.
