FIG. 1.

Sensitivities of the PCR assays for detecting HSV-1, HSV-2, and VZV. (A to C) Results of real-time PCR. Plasmids were serially diluted from 10⁴ to 10¹ copies per assay. In the lower left parts of the graphs, results for the corresponding no-template controls are shown. Vertical (y) axis, fluorescence intensity; horizontal (x) axis, PCR cycle numbers. (D) Results of nested PCR to detect HSV-1, HSV-2, and VZV. Lane 1, molecular weight marker (MWM); lanes 2 to 4, 10³ to 10¹ copies of HSV-1 plasmid controls; lanes 5 and 6, no-template control; lane 7, MWM; lanes 8 to 10, 10³ to 10¹ copies of HSV-2 plasmid controls; lanes 11 and 12, no-template control; lane 13, MWM; lanes 14 to 16, 10³ to 10¹ copies of VZV plasmid controls; lanes 17 and 18, no-template control.