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. 2000 Jan 18;97(2):680–685. doi: 10.1073/pnas.97.2.680

Figure 6.

Figure 6

Expression of Rab11 mutants alters the rate and extent of phagocytosis of EIgG. (A) Adherent RAW LR/FMLPR.2 cells transfected with the indicated constructs were incubated with EIgG at 4°C and the extent of binding was determined. (B) In parallel cultures, macrophages preincubated with EIgG at 4°C were incubated for varying times at 37°C to monitor the rate of phagocytosis in cells expressing either HA-Rab11 25N (■), HA-Rab11 70L (▴), or nonexpressing controls (●). Numbers in parentheses refer to percent of cell-associated EIgG ingested by 30 min. Data points represent mean ± SEM (n = 3). (C) Unsynchronized phagocytosis of EIgG was monitored in RAW LR/FMLPR.2 cells transfected with the indicated constructs. Percent phagocytosis of expressing cells compared with nonexpressing controls represents mean ± SEM (n = 3).