Table 1.

Kinetic parameters for rhodamine-transferrin recycling in RAW LR/FMLPR.2 cells expressing various Rab11 alleles

Transfected plasmid	Relative expression, %	kr (min−1)*	t1/2 (min)†
Mock (vector)		0.130  ±  0.011	4.9  ±  0.21
Rab11 WT	100  ±  8.6	0.120  ±  0.007	5.3  ±  0.68
Rab11 25N	102  ±  8.2	0.077  ±  0.010‡	9.1  ±  1.2‡
Rab11 70L	106  ±  7.7	0.231  ±  0.034‡	2.2  ±  0.70‡

Based on single-cell microspectrofluorometry. Each value represents mean ± SEM, n = 4. 

^*Rate constant for efflux of rhodamine-transferrin during 8 min of chase after 1 hr of loading. 

^† Amount of time required for efflux of 50% of total rhodamine-transferrin after 1 hr of loading. 

^‡P < 0.05 (Rab11 25N or Rab11 70L vs. mock-transfected).