Table 2.
Real Time PCR results (title and genotype) in comparison with cultural and conventional PCR methods using saliva and subgingival plaque from 10 Sardinian subjects out of 81, positive for A. actinomycetemcomitans.
| Patient | Disease type | Subgingival plaque | Saliva | |||
| Aa Real time PCR [cells/ml paper point] | Culture for Aa | Conventional PCR (for 4 periodontal pathogens) | Real time PCR Aa cells/ml | Aa Culture CFU/ml vs Conventional PCR (Aa 16S rRNA only) | ||
| C1 | aggressive periodontitis | 1,9*104 (652) | posit | Aa | 3,0*102 (652) | 1,5*101/pos |
| C2 | aggressive periodontitis | 4,0*103 (JP2) | posit | Aa, Pg | 1,3*102 (JP2) | neg/pos |
| C4 | gingivitis | 3,6*103 (652) | posit | Aa, | Nd | neg/neg |
| C5 | chronic periodontitis | 6,4*103 (JP2) | posit | Aa,Tf | Nd | neg/neg |
| C6 | chronic periodontitis | 1,9*103 (JP2) | neg | Aa,Tf | Nd | neg/neg |
| C7 | aggressive periodontitis | 2,8*104 (JP2) | posit | Aa,Pi,Tf | Nd | neg/neg |
| C8 | aggressive periodontitis | 4,4*104 (JP2) | posit | Aa, | Nd | neg/neg |
| C25 | aggressive periodontitis | 2,0*103 (JP2) | posit | Aa, | Nd | neg/neg |
| C75 | aggressive periodontitis | 3,0 *104 (652) | posit | Aa, Pg, Pi,Tf | Nd | neg/neg |
| C80 | no disease | 1,0*103 (652) | neg | Aa, Pg, Pi,Tf | Nd | neg/neg |
Legend:
Aa = A. actinomycetemcomitans, Pg = P. gingivalis, Pi = P. intermedia, Tf = T. forsythensis.
Nd = non-detectable (<102 cells/ml)