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List of primers used to amplify the rep-containing HdIV segments by Polymerase Chain Reaction
| HdIV segment | rep gene | forward primer (5'-3') | reverse primer (5'-3') |
| SH-A2 | rep7 | ATCTTAAAGTGAGCACTATTGACGC | CCTGCGAAATTTCTTGATACACCACAGCCT |
| SH-H | rep6 | AAGTGTTGCTTGACTCGGCT | TCAAGTCCAGGTTTCGGATC |
| SH-J | rep5 | CTTGGTTACTCCAGCCCTTG | ACTCCTCCGAATAAAGGCGT |
