Figure 2. IL-32 Induced by Inflammatory Stimuli Is a Cell-Associated Cytokine.

(A) Human PBMCs were stimulated with 10 μg/ml sonicated M. tuberculosis. After 24 h, supernatants and cell lysates were assayed for IL-32 (see Methods).

(B) RT-PCR of the four IL-32 isoforms in freshly lysed PBMCs (lane1), PBMCs incubated in medium for 4 h (lane 2), stimulated with 100 ng/ml LPS (lane 3), or with M. tuberculosis (lane 4).