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. 2006 Aug 15;3(8):e277. doi: 10.1371/journal.pmed.0030277

Figure 3. IL-32 Immunostaining of Human PBMCs Stimulated with LPS or M. tuberculosis .

Figure 3

(A) Freshly isolated PBMCs were left to adhere, stimulated with either control medium (first row), LPS (second row), or M. tuberculosis (MTB; third row), and 24 h later stained for the presence of IL-32. Nuclei were stained with AMCA (blue, left panels), and IL-32 was stained with Cy3 (red, middle panels). The right panels show the merged pictures with WGA-stained cellular surface (green).The stained cells were examined by digital deconvoluted microscopy.

(B) PBMCs stimulated with control medium (left panel), LPS (middle panel), or M. tuberculosis (right panel) were stained with an anti-IL-32 monoclonal antibody and counterstained with Mayer's hematoxylin for optical microscopy.