Figure 2.

Kinetics of ARP accumulation into intact IMR90 cells. IMR90 cells (3 × 10⁶/ml) in PBS/5 mM glucose were incubated with 3 mM ARP at 37°C for different intervals. At each time point, cells were sampled and processed as described in Materials and Methods, and the intracellular ARP was measured by HPLC-EC. The data is an average of three independent experiments (mean ± SD).