Table 2.

Assessment of the activity of 3-methyl adenine DNA glycosylase in senescent and young IMR90 cells

Young or senescent IMR90 cells (2 × 10⁶/ml) in PBS/5 mM glucose were treated with 3 mM ARP (Control), 3 mM ARP + 20 mM MMS for 1 h at 37°C. The cells were then washed in PBS, the DNA was isolated, and 1 μg of DNA was used for detection of AP sites as described. The values are mean ± SD. **, P < 0.01; ***, P < 0.001.