TABLE 3.
Comparison of 16S rRNA sequencing identification with phenotypic identification
| Phenotypic identification (n) | 16S rRNA sequencing identificationa (% identity) in comparison with:
|
No. matched (%)b | |||
|---|---|---|---|---|---|
| Type strain sequence (% identity) | n | GenBank type strain sequence (% identity) | n | ||
| Anaerococcus prevotii (20) | Anaerococcus prevotii (99.5) | 1 | Uncultured clone KL-59-7-12 (99.3) | 1 | 1 (5) |
| Peptoniphilus harei (>99.0) | 18 | Peptoniphilus harei (> 99.0) | 18 | ||
| Peptoniphilus asaccharolyticus (91.7) | 1 | Peptostreptococcus sp. (93.8) | 1 | ||
| Anaerococcus tetradius (10) | Anaerococcus tetradius (99.9) | 3 | Anaerococcus tetradius (97.4) | 3 | 3 (30) |
| Anaerococcus vaginalis (99.9) | 6 | Anaerococcus vaginalis (97.2) | 6 | ||
| Finegoldia magna (88.7) | 1 | Finegoldia magna (86.9) | 1 | ||
| Finegoldia magna (36) | Finegoldia magna (100) | 36 | Finegoldia magna (98.0) | 36 | 36 (100) |
| Micromonas micros (33) | Micromonas micros (99.8) | 33 | Peptostreptococcus sp. oral clone FG 014 (99.9) | 33 | 33 (100) |
| Peptostreptococcus anaerobius (27) | Peptostreptococcus anaerobius (99.8) | 15 | Peptostreptococcus anaerobius (99.8) | 15 | 15 (55.6) |
| Peptostreptococcus anaerobius (98.0) | 12 | Peptostreptococcus sp. oral clone CK 035 (99.9) | 12 | ||
| Peptoniphilus asaccharolyticus (30) | Peptoniphilus harei (>99.0) | 19 | Peptoniphilus harei (>99.0) | 19 | 0 (0) |
| Peptoniphilus harei (97.2) | 2 | Peptoniphilus harei (97.2) | 2 | ||
| Peptoniphilus harei (95.6) | 3 | Peptoniphilus harei (95.6) | 3 | ||
| Peptoniphilus harei (93.2) | 2 | Peptoniphilus harei (93.2) | 2 | ||
| Peptoniphilus ivorii (84.8) | 1 | Peptoniphilusgenospecies 4 (89.2) | 1 | ||
| Micromonas micros (83.9) | 1 | Micromonas micros (88.4) | 1 | ||
| Anaerococcus octavius (95.5) | 2 | Anaerococcus octavius (95.5) | 2 | ||
| Total | 88 (56.4) | ||||
a
Possible novel species are in boldface.
b
That is, the number of matches between the phenotypic identification and the 16S rRNA sequencing identification based on type strain sequences determined in this study.