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. 2006 Aug;13(8):898–904. doi: 10.1128/CVI.00056-06

FIG. 1.

FIG. 1.

Expression of the ESAT-6 antigen in Vero cells infected with the recombinant influenza virus vectors. (a) Diagram of the recombinant NS1 protein expressed by influenza A vectors. (b) Western blot of lysates of Vero cells infected at an MOI of 2 with recombinant Flu/ESAT-6 viruses of different subtypes (H1N1, H2N2, and H3N2), using monoclonal antibodies to ESAT-6. (c) Expression of the ESAT-6 protein in the nuclei of infected cells, shown for the Flu/ESAT-6 (H1N1) vector by immunofluorescence using polyclonal serum to ESAT-6.