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Design of plasmid constructs and expressed proteins. A 595-bp SalI/EcoRI fragment of DuCD154 was inserted into pSecTag2C (ptCD154) or was fused with a 512-bp EcoRV/HincII fragment of the DHBV core in pSecTag2C (ptcore-tCD154). The EcoRV-HincII fragment alone was also ligated into pSecTag2C (ptcore). aa, amino acids.
