Comparison of mean antibody titers of immunized groups. Mean endpoint titers ± standard errors are indicated for ducks immunized with ptcore-tCD154 (□), ptcore (⧫), ptcore plus ptCD154 (○), or pSecTag2C (▴). Statistical comparisons at the indicated time points were between ptcore-tCD154- and ptcore-immunized groups (*) or between ptcore-tCD154- and ptcore plus ptCD154-immunized groups (+). * and +, P ≤ 0.05; ++, P ≤ 0.01; +++, P ≤ 0.005.