FIG. 5.

Diagnostic specificity and sensitivity of EVELISA. M. avium subsp. paratuberculosis-negative serum samples were obtained from cattle on dairy farms that had tested JD negative by ELISA for 5 years and by fecal culture, PCR, gamma interferon tests, and ELISAs performed during the fifth year. M. avium subsp. paratuberculosis (MAP)-positive serum samples were obtained from cattle whose feces resulted in formation of more than 4 colonies by the fecal culture test. A cutoff value of 0.24 was used to distinguish M. avium subsp. paratuberculosis-negative and -positive results. Similar results were obtained in three separate experiments.