FIG. 6.

Effect of overexpression of DhGZF3 on Gln3- and Gat1-deficient cells. Wild-type strain BY4741 (WT) and its isogenic gln3 and gat1 derivatives were transformed with YEplac181 (−) or YEp-DhGZF3 (+). Colonies were recovered from transformation plates lacking Leu (−Leu) (very small in the case of gln3 transformants) and grown overnight on liquid medium lacking Leu. Cells were then inoculated into YPD medium and grown overnight. Finally, dilutions were spotted onto the indicated plates and incubated for 48 h. CM, complete medium.